Changes in steroid receptor number of and bioactivity of gonadotropin in the follicular fluid of porcine ovarian atretic follicles, 1; Bioavailable testosterone
1989
Yoon, Y.D. | Lee, C.J. | Chun, E.H. (Hanyang Univ., Seoul (Korea Republic). Coll. of Natural Sciences) | Lee, J.Y. (Chungbuk National Univ., Chungjoo (Korea Republic). Coll. of Agriculture)
The present study was designed to determine the concentration of bioavailable steroid hormones in the atretic follicular fluid (FF). The concentrations of progesterone (P), testosterone (T), estradiol (E), androstenedione (A), and 5-alpha dihydrotestosterone (DHT) were determined by the established methods of luminescent immunoassay (LIA) or radioimmunoassay (RIA). Concentrations of T, A and DHT in human FF from small ( 6 mm), medium (8-15 mm), and large ( 15 mm) atretic follicles were significantly higher than those of normal ones (p 0.01). However, the levels of T, A and DHT in small atretic follicle were significantly lower than those found in normal one. The concentrations of P in atretic FF from porcine small ( 3 mm), medium (4-6 mm), and large ( 7 mm) follicles were not different from that of normal ones. However, the concentration of E in atretic follicles of each group was significantly lower than that of normal group (p 0.001 in each group). On the other hand, the percentages of bioavailable T (BT) in human FF were significantly (p 0.001) higher than those in normal groups. The BT in normal or atretic FF was more than 90 % of total T. The present result demonstrates that the bioavailable androgen, but not E levels in atretic follicles is higher than that of normal one, and that the atretic mechanism might be dependent on the ovarian follicle size in the developmental stage and on the animal model system. Moreover, the present study suggests that the steroids found in the FF are the bioavailable forms and the concentration of BT in FF could be used as one of the valuable criteria classifying the ovarian atretic follicle
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