Comparison of methods for monitoring green leafhopper, Nephotettix virescens (Distant), feeding during tungro transmission [Philippines]
1988
Dahal, G. | Hibino, H. | Saxena, R.C. (International Rice Research Inst., Los Banos, Laguna (Philippines))
A comparison was made of the honeydew tests using bromocresol, ninhydrin, or safranin dye and an electronic device for monitoring feeding of viruliferous Nephotettix virescens adult during inoculation access and transmission of rice tungro bacilliform (RTBV) and rice tungro spherical (RTSV) viruses in seedlings of vector and/or disease resistant rice cultivars. Inoculated seedlings were indexed for virus infection by ELISA [Enzyme Linked Immunosorbent Assay]. Electronic recording and bromocresol tests were appropriate for determining the relationship between feeding sites and virus transmission. The ninhydrin test was good for monitoring phloem feeding, but not for non-phloem feeding. The safranin test accurately determined xylem feeding, but treated seedlings could not be used for virus assay. Electronic monitoring of insect feeding was accurate, but only one or two individuals could be tested at a time with one recorder. The bromocresol method could be used for monitoring feeding by many individuals simultaneously, although it was less accurate as the honeydew spots excreted on the filter paper disks sometime overlapped.
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