Effect of temperature and some media and biotic factors on the growth of Fusarium oxysproum f. sp. lentis, and its mode of seed transmission
1990
Erskin, W. (International Center for Agricultural Research in the Dry Areas - ICARDA, Aleppo (Syria)) | Bayaa, B. | Dholli, M.
This study was conducted to determine the effects of some factors, namely temperature (10, 15, 20, 25 and 30 deg C) and media (potato dextrose agar, lentil dextrose agar and (Czapek), on the growth in vitro of Fusarium oxysporum f. sp. lentis in order to produce sufficient inoculum for the development of a screening technique, as well as to clarify such aspects of disease epidemiology as mode of seed transmission and association with nematodes and antagonistic bacteria. The optimum temperature for fungal growth was 22 deg C. Maximum mycelial growth and sporulation were obtained on lentil dextrose agar. In vitro studies revealed an antagonistic effect between the fungus and a Pseudomonas sp. isolated from infected soil. The following nematode genera were associated with wilt in the field: Ditylenchus dipsaci, Aphelenchoides spp., Aphelenchus spp., Helicotylenchus spp., Heterodera spp., Meloidogyne spp., Pratylenchus spp. and Tylenchorhynchus spp. with the former being the most prevalent. The fungus was not present either in the endosperm or under the seed coat of seed from a crop showing wilt symptoms
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