Control of sclerotium seedling blight of barley by using seeds coated with Trichoderma spp. and Bacillus sp.
1992
Chamswarng, C. | Gesnara, W. | Korpraditskul (Kaesetsart Univ., Nakhon Pathom (Thailand). Dept. of Plant Pathology)
The efficacy of six isolates of Trichoderma spp. and one isolate of Bacillus sp. for the control of seedling blight on barley (BRB 2) caused by Sclerotium rolfsii Sacc. was investigated under screenhouse conditions. Barley seeds were coated with spore/cell suspension alone or in combination with test isolates. Seeds coated with each spore suspension of T. harzianum (T1, T2) and Trichoderma sp. (T9) or as combinations of T. harzianum (T1) with Bacillus sp. (B1), T. aureoviride (T4) with Bacillus sp. (B1) or T. longibrachiatum (T7) were significantly protected from sclerotium infection with 30.0, 25.0, 45.0, 30.0, 27.5 and 25.0 more surviving plants than the control. These control capabilities were not significantly different from the carboxin when compared with the control. Twenty eight days after planting, populations of these Trichoderma isolates in the rhizosphere of treated seeds were found ranging from 3.5 to 6.0 x 10 7 cfu/g soil. Replanting of surface-disinfested barley seeds in soil formerly planted with treated seeds (Trichoderma spp. and/or Bacillus) showed that numbers of surviving plants of all treatments except seeds treated with T. longibrachiatum (T7) and T. aureoviride (T4) in combination with Bacillus sp. (B) were significantly higher than the control and not significantly different from the carboxin treatment
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