Mutation breeding technique in tissue culture of carnation by gamma irradiation
1984
Oradee Sahavacharin | Chaichoompon Suriyasak (Kasetsart Univ., Bangkok (Thailand). Faculty of Agriculture. Dept. of Horticulture)
Shoot tips of 'White Sim' carnation were aseptic cultured in different media for shoot formation. The highest shoot formation was obtained as high as 80 % in Murashige and Skoog plus 15 % coconut water media. With the same media plantlets produced multiple shoots for further experiment. Each shoot tip of about 1.5-2 cm long was cultured in Murashige and Skoog media for gamma-ray irradiation studies. With the increasing dose, the survival as well as growth rate were decreased. Shoots died 12 weeks after gamma-ray treatment at the dose of 7 krad. When plantlets were transplanted into soil, the survival rate decreased with the increasing dose and they all died at 5 krad treatment. Then the shoots from axillary bud of VM1 plant were cut and transplanted into soil to study in VM2 plant. Vm2 plants were multiplied once again through axillary side shoots cutting to produce VM3 plants. The diameter of the flowers and size of leaves were not different from the control. When meristem tips, size of stomata and chromosome number from root tips were studied, there were no significant differences from the control. The irradiated plants at 1 krad and 3 krad differed from the control by having the pink streak on white petals of the flowers.
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