Effects of zinc deficiency on peptide synthesis in cultured tobacco [Nicotiana tabacum] plant cells
1994
Obata, H. (Mie Univ., Tsu (Japan). Faculty of Bioresources) | Toda, T. | Umebayashi, M.
To analyze the effects of Zn deficiency on protein synthesis at the translation and transcription levels, proteins and peptides extracted from tobacco cells cultured with (+Zn) and without Zn (-Zn) were compared by two-dimensional electrophoresis, mRNAs were compared by in vitro translation. Although the spots of translated peptides were almost identical for the -Zn and +Zn treatments, one peptide spot at pI 6.0 appeared densely by the -Zn treatment. Clear differences were observed in the separation patterns of water soluble cellular proteins and peptides. In the -Zn treatment, two peptides at pI 4.5 (MW 45,000) and at pI 5.5 (MW 50,000) which were present in the +Zn treatment disappeared while one appeared at pI 6.0 (ME 28,000). Since may participate in the formation of some peptides and under Zn deficiency, the transcription of one mRNA for a peptide which appeared at pI 6.0 was enhanced in this study
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