Production and specificity of monoclonal antibodies reactive to progesterone [surveying with rabbit]
1994
Kang, C.B. (National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)) | Domeki, I. | Kamomae, H. | Ikeda, T. | Yukawa, M. | Onodera, T.
Monoclonal antibodies to progesterone 11alpha-hydroxy hemisuccinate were prepared. Supernatant of clone hybridoma reacted positively to the progesterone solution. Dot-blot analysis revealed a single precipitation band when rabbit anti-mouse IgM and anti-mouse kappa were used. These monoclonal antibodies did not react with pregnenolone, testosterone, estrone, estradiol-17beta, aldosterone, hydrocortisone (cortisol), cortisone, corticosterone and 11alpha-dehydroxycortisone (DOC). Radioimmunoassay using those monoclonal antibodies was developed. Data from this assay supported the following conclusions: (1) association constant (KA) of supernatant of clone PrM for progesterone was 2 x 10(10), (2) monoclonal antibodies reacted to progesterone most efficiently at 1:10,000 dilution, and (3) efficiency of reactivity of monoclonal antibodies was higher than that of rabbit antibodies
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