Efficacy of three fungicides for controlling growth of five seedborne fungi associated with rice grain spotting
1994
Sisterna, M. | Ronco, L. (Comision de Investigaciones Cientificas, Buenos Aires (Argentina))
Ten fungi were isolated from field-infected panicles using the blotter test method, following the rules of the International Seed Testing Association. The following were selected for an in vitro control test due to their importance and frequency; Bipolaris oryzae, Curvularia lunata, an unidentified species, and Fusarium semitectum. Fusarium moniliforme was included as an ordinary pathogenic control species. Thiabendazole, Carbendazim, and Mancozeb fungicides were used. Solutions were prepared using 50, 100, 500, and 1,000 ppm sterile distilled water. For each, 1 ml of the fungicide solution was mixed with glucose potato agar (GPA), melted at 50 deg C, and poured in petri dishes. Agar slugs (6-mm-diam disks) of each species were then centered in the dishes containing the agar and fungicide concentrations. Each concentration was replicated four times as was the control sample (0 ppm) in which 1 ml distilled water was added to the melted agar. Petri dishes were kept on an incubator at 25 deg C. Colony diameters were measured on days 2, 4, 6, 8, and 10. Of the three products, mancozeb had the greatest efficacy on B. oryzae and C. lunata, with the greatest effect at 500 ppm. Carbendazim and thiabendazole had good efficacy at 50 ppm in controlling the growth of F. semitectum, F. moniliforme, and the unidentified species
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