Productivity of somatic embryos in crossed progenies from regenerable and non-regenerable alfalfa (Medicago sativa L.) genotypes
1995
Okumura, K. (National Grassland Research Inst., Nishinasuno, Tochigi (Japan)) | Gau, M. | Kanbe, M. | Takai, T. | Matsuura, M.
Variation in productivity of somatic embryos was investigated in the progeny populations from a male sterile genotype,MS, of alfalfa (Medicago sativa L.). The three traits, number of somatic embryos, stability of embryo formation and days to embryo formation, were referred to as the productivity of somatic embryos in this study. The progeny populations from the crosses between MS and four male fertile pollen parents, regardless of their regeneration ability, showed more somatic embryo production than in MS itself which showed low productivity of embryos. Six genotypes were selected from the crossed progeny populations according to their productivity of somatic embryos. For further analysis, these genotypes were crossed with two genotypes, and one had the regeneration ability and the other had no regeneration ability. In average through each pollen parental genotype the progeny populations from the regenerable pollen parent were superior to those from the non-regenerable genotype for productivity of somatic embryos as well as regeneration frequency. However, one of the six genotypes showed superior embryo production in the crossed progeny population with the non-regenerable genotype parent to that with the regenerable genotype
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