A simple method of laboratory silage fermentation by using a plastic pouch for packaging
1995
Tanaka, O. (National Grassland Research Inst., Nishinasuno, Tochigi (Japan)) | Ohmomo, S.
To develop a simple method of laboratory silage fermentation, we made several experiments of ensiling and a model system of ensilage by use of milled alfalfa hay cube added with water (AHC medium) and inoculated with a lactic acid bacterium (LAB), a butyric acid bacterium (BAB) and a coliform bacterium (CFB). In these experiments, we used plastic pouches for anaerobic incubation instead of bottle silos and anaerobic jars and the following results were obtained. 1) The quality of the silage in the pouch was almost the same as that in the bottle silo when one lot of alfalfa was used as a plant material. 2) The quality of the model system of ensilage was reproducible if incubation conditions were consistent. The standard deviation of that was below +-9%. Butyric acid was produced when the moisture content of AHC medium was 85%, but no butyric acid was produced when the moisture content of the medium was reduced to 70%. 3) When the moisture content of AHC medium was 85%, low soluble sugar content of the medium (less than 0.5%) and smaller inoculum size of LAB than that of CFB were effective for butyric fermentation. Under these conditions, multiplication CFB was observed
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