Characterization of strains of Bemisia tabaci (Gennadius) using molecular biology techniques | Caracterisation de souches de Bemisia tabaci (Gennadius) par des techniques de biologie moleculaire
1997
Menozzi, P. (CIRAD-CA, Montpellier (France))
The increase over the past ten years in problems related to Bemisia tabaci results to a considerable extend from a more aggressive biotype that appears to have spread steadily throughout the world. Some biological characteristics of the biotype have led some entomologists to raise this biotype to the rank of a new species called Bemisia argentifolii Bellows & Perring. Random amplification of some parts of the genome using RAPD-PCR (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) was used to identify samples of adults from three countries in Africa (Benin, Cameroon and Ivory Coast) and one in Central America (Nicaragua) collected on different host plants. In comparison with enzymatic analysis, these molecular biology techniques have the advantage of enabling easy distinction between very close species or strains. Analysis of the amplification patterns using the UPGMA method (Unweighted Pair-Group Method for arithmetic Averages) revealed the existence of several biotypes according to the nature of the host plant and the geographical location.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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