Relationship between ruminal endotoxin level and number of bacteria in goats engorged high-concentrate ration
1997
Suda, K. (Kaken Pharmaceutical Co. Ltd., Tokyo (Japan)) | Yoshioka, M. | Motoi, Y. | Kobayashi, Y. | Wakita, M. | Hoshino, S.
The present study aimed to investigate the relationship between ruminal endotoxin (ET) level and number of bacteria in goats engorged a high-concentrate ration. Three hay-fed goats fitted with ruminal fistula were voluntarily fed with a 90% concentrate ration in order to induce acidosis. Clinical signs such as anorexia or depression and significant decline of ruminal pH accompanied with an elevation of VFA level were observed in all goats soon after the change of diet. Molar percentages of acetate and iso-valerianate decreased and that of propionate increased from 6 to 48 h. There were no changes in ruminal L(+)- and D(-)-lactate levels. Ruminal ET level, however, gradually elevated from 6 h and, after reaching a maximum level of 167.7 ng/ml at 24 h, declined to the initial level at 168 h. Both numbers of gram-positive and -negative bacteria tended to slightly increase at 24 h. The proportion of gram-positive cocci tended to increase, and that of gram-negative cocci significantly decreased at 6 and 12 h. The significant decrease in total number of protozoa was observed from 6 to 24 h and 72 h. It was clarified that ruminal ET production increased without a decrease in number of gram-negative bacteria, when ruminal fermentation was activated
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