Enumeration and lsolation of the herbicide atrazine degraders from soils
1993
Roongnapa Korpraditskul | Katayama, A. | Kuwatsuka, S. (Kasetsart Univ. Kamphaengsaen Campus, Nakhon Pathom (Thailand). Kasetsart Univ. Research and Development Inst. Central Lab. and Greenhouse Complex Center. Environmental Science Unit)
Herbicide atrazine has been intensively and indiscriminately used for weed control in sugarcane, corn and other field crops in Thailand. Althoug it is known that atrazine was degraded through chemical and biological processes in soils, atrazine was occasionally detected in surface water and drinking water in U.S.A. and other countries. Degradation of xenobiotic compounds by soil microorganisms is an important methods to rehabilitate the environmental contamination problems. The procedure to enumerate the population of soil microorganisms which able to degrade atrazine was carried out by the Most Probable Number (MPN) method. The criteria of the atrazinedegrading ability of the microorganisms in soils was set at 20 percent of atrazine lost from initial concentration within 30 days of incubation. Atrazine-degrading microorganisms were isolated from MPN tubes. Anjo soil from Japan harbored 2.1*10** (6) cells/g soil of atrazine degraders while Pak Chong soil from Thailand harbored 4.5*10** (5) cells/g soil. Among the dominant microorganisms from MPN tubes, 16 isolates were obtained from both Pak Chong and Anjo soil.
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