Production of gibberellin from Gibberella fujikuroi by solid state fermentation
1992
Jaruwan Kovsurat
The important fungi for industrial gibberellin production is Gibberlla Fujikuroi. Fourteen strains of Gibberella fujikuroi were screened to select for the most potent strain. Gibberella fujikuroi TISTR 3394 was the strain that gave maximum production of gibberellic acid (GA3), which is the most important gibberelin being used extensively in agriculture. Studies on optimization of GA3 production in 25 grams of wheat bran solid substrate, using 500 ml Erlenmayer flask, showed that Gibberella fujikuroi TISTR 3394 produced the highest quantity of GA3 at 50 % initial moisture content of wheat bran substrate without pH adjustment (pH 6.0) incubated at 30+-1 deg C for 6 days. The value of maximum GA3 produced was 969 mg/kg dry weight of fermented substrate. For GA3 production in rice bran solid substrate, each containing 25 grams rice bran in 500 ml Erlenmayer flask, the following conditions were chosen as optimum: 60 % initial moisture content of rice bran medium without pH adjustment (pH 5.4) and incubated at 30+-1 deg C for 8 days. Gibberella fujikuroi TISTR 3394 produced the maximum GA3 at about 921 mg/kg dry weight of fermented substrate. The study on the effect of different solvents on the extraction capability of GA3 produced by Gibberella fujikuroi TISTR 3394 from rice bran and wheat bran fermented substrate was performed. Three kinds of solvents were compared methanol, ethanol and acetone at 10 % (v/v) in aqueous solution, using ethyl acetate as control. Among the various solvents tested, 10 % (v/v) ethanol was found to be the best solvent with the highest extraction capacity. The degree of extraction of GA3 decreased in respective order by using 10 % (v/v) acetone, 10 % (v/v) methanol and ethyl acetate as solvent.
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