Bacterial and fungal immobilization on PAA hydrogels usable in cellulose biodegradation
1997
Petre, M. (Research Centre for Ecological Technologies, Bucharest (Romania)) | Zarnea, G. | Adrian, P. | Gheorghiu, E. | Bartha, C.
This paper refers to some methods concerning the immobilization of bacterial and fungal cells on polyacrylamide (PAA) hydrogels, used in cellulose biodegradation. The experiments were achieved in a biotechnological laboratory-scale installation for continuous cultures of bacteria and fungi. The fungal development on polyacrylamide hydrogels compared with that one on agar hydrogel, in the same culture conditions, emphasizes the importance of physical and chemical structure of any support used in cellular immobilization. The immobilization of bacterial cells was achieved using Bacillus subtilis strains, which were immobilized on simple PAA hydrogel 20 per cent and mixed PAA hydrogels with Collagen 1 per cent, 3 per cent and 5 per cent. The immobilization of Pleurotus ostreatus as vegetative mycelium was performed in simple PAA hydrogels 10 per cent, 15 per cent and 20 per cent. Trichoderma viride was immobilized in simple PAA hydrogel 20 per cent and mixed PAA hydrogels 20 per cent with Collagen 1 per cent, 2 per cent, 3 per cent. The agar hydrogels 3 per cent were used in all experiments as control samples. The best rate of cellulose hiode gradation was achieved by immobilization of viable bacterial cells in mixed PAA hydrogels 20 per cent with Collagen 3 per cent and by vegetative mycelial immobilization of macroand microfungi in simple PAA hydrogels 20 per cent and mixed PAA hydrogels with Collagen 1 per cent.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل University of Liège