Genetic diversity in sweetpotato (Ipomoea batatas L.) and yam (Dioscorea sp.)
1999
Anselmo, B.A. | Ganga, Z.N. | Heimer, Y.M. | Nejidat, A. (Benguet State Univ. La Trinidad, Benguet (Philippines))
Genetic diversity analysis was performed in sweetpotato genotypes from the Philippine highlands and Discorea germplasm from Nigeria, Kenya and Philippines. Genomic DNA from seven potato genotypes selected from those which produced storage roots under drought conditions in the Northern Philippine highlands was subjected to Random Amplified Polymorphic DNA (RAPD) analysis. Twenty decamer primers were tested and 16 yielded DNA-amplification products. Among the 97 scored bands, 77 were polymorphic (79.38 percent) and genotype specific DNA markers were also identified. The pairwise marker difference between genotypes ranged from 0.378 to 0.936 indicating a broad range of genetic diversity. RAPD analysis was likewise done in 16 clones belonging to 3 species of yam, D. bulbifera, D. rotundata and D. alata. Twelve primers yielded DNA-amplification products in all yam species and clones. Of the 84 bands generated, 98.8 percent were polymorphic. Coefficient of similarity ranged from 0.45 to 0.98 for closely related clones into three separate groups show that RAPD markers can efficiently discriminate the different clones in a yam germplasm of diverse origin. The D. bulbifera group originated in Kenya, the D. rotundata group in Nigeria and "Sampero" in the Philippines
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