Efficacy of Romet-30 against vibriosis infection in black tiger shrimp (Penaeus monodon Fabricius)
1995
Jatiya Rochanasiri
Efficiency of Romet-30 against vibriosis infection in black tiger shrimp (Penaeus monodon) determined. The sensitivity of Vibrio spp. isolated from deseased shrimp was studied. Minimal Inhibitory Concentration(MIC) of Romet-30 to twenty strains of bacteria was found to be between 0.5-2.0 ppt. Shrimp were fed with medicated (Romet-30) pellet feed at doserates of 2 g/kg, 3.3 g/kg of feed for five days prior to intramuscular injection with V. parahaemolyticus at the LD50 level (3.16 * 10*[6) cells). Survival rate was observed for seven days. Shrimp fed with a high dose of medicated feed had a higher survival rate (55 percent) than the low dose (30 percent) and control (25 percent). Histopathological changes, at 1 hour post injection, control and pre-medicated groups showed a lower concentration haemocytes in damaged tissue than the non-medicated group. Twenty four hours post infection, the control group showed the smallest area and damage. The non-medicated group showed more extensively damaged tissue than pre-medicated shrimp. Seven days post injection, an affected area in the control was almost healed, whereas large damaged areas with dense haemocytes were found in the bacterial injected group and these healed more slowly than the medicated group.
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