Effect of formaldehyde on the growth and protein biosynthesis of Candida didensii 74-10 in transient regimes under nitrogen limitation
2000
Tzvetkova, B. | Katranushkova, Ch. | Losseva, L. | Hristozova, Ts. (Bulgarian Academy of Sciences, Institute of Microbiology, BG-1113 Sofia (Bulgaria))
We carried out our investigation on strain C. didensii 74-10 under continous cultivation, nitrogen limitation and dilution rate of 0.1 h-1 after pulse on the yeast populations with 0.01%, 0.02% or 0.04% formaldehyde. The results showed that during the transient regime after pulse with 0.02% formaldehyde it was observed changes in biomass and protein content. At a new steady state the values of the main parameters reached those of the control culture without inhibitor. The data showed an increase of activities of NADPH- and NADH-glutamate dehydrogenase. It was found that under nitrogen limitation after pulse with 0.04% formaldehyde the cell growth was extremely inhibited, biomass and protein yields were sharply decreased, and the culture could not rech a new steady state, because of unability to get over the inhibiting impact of the formaldehyde.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institute of Agricultural Economics