Restriction cleavage analysis of MtDNA extracted from the four conserved goat in Yunnan Province, China
1998
Ye Shaohui | Peng Helu(Yunnan Agricultural Uni., Kunming, Yunnan (China). College of Animal Science and Technology)
Mitochondral DNA abstracted from Yunnan four conserved goats distributed in different regions of Yunnan Province, China had been digested with twenty restriction endonucleases: Apa I, Ava I, BamH I, Bcl I, Bgl I, Bgl II, Cla I, Dra I, EcoR I, EcoR V, Hae I, Hind III, Kpn I, Pst I, Pvu II, Sac I, Sal I, Sma I, Stu I and Xho I. The molecular weight of the mtDNA was about 15.8 kb. The different restriction endonucleases broke mtDNA into different sizes. Digestion with BamH I, Stu I and EcoR I produced five fragments respectively, four with Hind III, three with Pvu II, Bgl II and Sca I, respectively, two with Apa I, Ava I, Cla I, Hpa I and Pst I, respectively. These primary results may present some useful data for the study on the evolution and differentiation of the conserved goats in Yunnan Province, China.
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