The responses of ATPase and PPase activities and lipid composition of tonoplast in roots of two wheat cultivars to aluminium stress
1999
He Longfei | Shen Zhenguo | Liu Youliang (Nanjing Agricultural Univ. (China). Dept. of Agronomy)
This paper dealt with the changes in ATPase and PPase activities, lipid composition of tonoplast vesicles from two wheat (Triticum aestivum) cultivars, Altas 66 (Al-tolerant) and Scout 66 (Al-sensitive) under Al stress. In comparison with the controls, the activities of H+-ATPase and Ca2+-ATPase of tonoplast vesicles in Altas 66 were decreased greatly by AlCl3 20 and 100 mumol/L treatment, those of Scout 66 were increased at AlCl3 20 mumol/L, but it decreased at AlCl3 100 mumol/L (Figs. 1, 2). The H+-PPase activity of Altas 66 was decreased by Al treatment, while that of Scout 66 increased (Fig. 3). The phospholipid contents of tonoplast vesicles in Altas 66 and Scout 66 were increased by AlCl3 20 mumol/L treatment, but it was decreased by AlCl3 100 mumol/L (Fig. 4). The contents of galactose in lipids of two cultivars after Al treatments was higher than those of the controls, and it was higher in Altas 66 than in Scout 66 (Fig. 5). After Al treatment, the palmitic acid and stearic acid contents increased, the linolenic acid content and the unsaturation index of fatty acids decreased in both cultivars, but those in Scout 66 more distinctly (Table 3). To sum up, the tonoplast vesicles of roots of Altas 66 showed a higher adaptability to Al stress than that of Scout 66
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