Effect of seminal plasma on the storage of buck semen

A study was conducted to examine the effects of washing goat spermatozoa, substituting goat seminal plasma with boar seminal plasma, and incorporating or removing seminal plasma in siluents for liquid storage of goat spermatozoa under nitrogen atmosphere at 5 deg C. This experiment was of 2 x 3 factorial design and incorporated in the following factors: Factor A involved washed and unwashed spermatozoa while Factor B consisted of three types of diluents such as Boar Seminal Plasma + Tris-Fructose-Eggyolk + Nitrogen (Vacuum + flush method) (TFY-VFN + BSP), Goat Seminal Plasma + Tris-Frustose-Egg Yolk + Nitrogen (Vacuum + flush method) (TFY-VFN + GSP), the Boar Seminal Plasma. Each treatment was replicated 3 times and motility was assessed after 0, 1, 2, 3, 4 days storage while acrosome integrity was assessed on days 0 and 4. Smears were prepared and stained and cleared using Naphthol yellow S and Erythocin B. A repeated measures analysis of variance for split-plot design with the storage time as the subplot factor using orthogonal polynomial coefficients on effects of time. Dadiangas strain and upgrade Anglo-nubian bucks were trained to serve the artificial vagina for semen collection. Their semen were pooled before processing at 30 deg C in a waterbath. A trained Duroc boar was used as donor of seminal plasma. A method of centrifugation to separate seminal plasma from spermatozoa in boar semen and washed goat spermatozoa was used. Best survival (P0.001) of goat spermatozoa was shown in TFY-VFN added with 50 percent BSP in washed spermatozoa, whereas TFY-VFN + GSP has higher motility in washed spermatozoa but has comparable effect with unwashed spermatozoa. A pure BSP diluted to either washed or unwashed goat spermatozoa has no beneficial effect. Washing of goat spermatozoa showed a significant effect (P0.05) than unwashed spermatozoa. Motility of liquid-stored spermatozoa declined with increasing storage time (linear, P0.001). High percentage (P0.001) of intact acrosome either washed or unwashed spermatozoa was observed when diluted with TFY-VFN + BSP liquid-stored at 5 deg C under nitrogen atmosphere. This was comparable to the TFY-VFN + GSP which has greater proportion (P0.001) of normal acrosomes in washed than in unwashed spermatozoa. BSP alone gave the lowest percentage of intact acrosomes. This result further signifies that TFY-VFN + BSP extender can now be used as an alternative extender for liquid storage of goat spermatozoa for economic reasons