Plasma free amono acid fractions in different phases of reproduction in dwarf nanny goat

One dimensional thin layer chromatography was employed to detect free amino acids fractions in various phases of reproduction in dwarf goat. Cystine of band 1, observed in amino acids standard, could not be separated in samples. No appreciable variation was observed in arginine, histidine, lysine, glutamine and asparagine fractions of band 2, tryptophan, valine and methionine of band 7 and phenylalanine and 3, 4-dihydroxyphenylalanine of band 8 throughout the estrous cycle. Glycine and serine of band 3 were significantly reduced, compared to proestrus, in estrus, metestrus and diestrus phases. Threonine of band 4 and leucine and iso-leucine of band 9 were significantly enhanced in estrus compared to proestrus phase. The level was however, restored in succeeding phases of estrous cycle. Alanine of band 5 and tyrosine of band 6 were markedly reduced in estrus compared to proestrus phase. The level was found rehabilitated in following metestrus and diestrus stages. Arginine, histidine, lysine, glutamine and asparagine of band 2 were found to be markedly enhanced in early, mid and late gestation compared to pre-pregnancy estrus. Glycine and serine of band 3 were significantly depressed in mid and late gestation compared to pre- pregnancy estrus. Threonine of band 4 appreciably declined in early but markedly enhanced in mid and late gestation compared to pre-pregnancy estrus. Tyrosine of band 6 remained almost unaffected in early and mid however, reduced in late gestation in comparison with pre-pregnancy estrus. Tryptophan, valine and methionine of band 7, phenylalanine and 3, 4-dihydroxyphenylalanine of band 8 and leucine and iso-leucine of band 9 remained almost in the range of pre-pregnancy estrus throughout the entire course of pregnancy. Arginine, histidine, lysine, glutamine and asparagine of band 2 and threonine of band 4 were appreciably enhanced in mid and late compared to early lactation. Glycine and serine of band 3 reduced in mid and late but elevated in advanced lactational phase compared to early lactation. Alanine of band 5 and tryptophan, valine and methionine of band 7 depressed significantly in mid and late lactational phases in comparison with early lactation. Tyrosine of band 6 reduced markedly in late compared to early lactation. No significant variation was observed in phenylalanine and 3, 4- dihydroxyphenylalanine of band 8 and leucine and iso-leucine of band 9 throughout the entire course of lactation.