Molecular approach to identifying linkage groups in Saccharum species and hybrids using maize DNA probes
1995
Glaszmann, C.J. | Lu, H.Y. | D'Hont, A. (CIRAD, IRAT, BP 5035, 34032 Montpellier (France))
Modern sugarcane varieties are complex aneuploids and typically have chromosome numbers in the 100-125 range with about 5-10 percent of them contributed by wild relatives, mainly Saccharum spontaneum and the rest by S. officinarum. Due to such genetic peculiarities, molecular genetic markers cannot be applied to sugarcane as they are to most other plants. A specific approach was suggested by isozyme studies. The comparison of S. spontaneum and S. officinarum revealed a high number of bands specific to the former species. This showed that the largest part of the diversity among sugarcane varieties is related to the presence/absence of S. spontaneum genes. Moreover, a linkage between two such genes could be detected in the self progeny of a variety where a single S. spontaneum chromosome bore unique alleles for the two genes. We pursued similar studies with RFLPs, using maize probes whose location on the maize genetic map is known. We thus intended to test a plausible similarity among members of the Andropogoneae tribe, as was suggested by the large homosequential portions observed between the genomes of maize and sorghum.
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