Fluorescent AFLP detection of dwarf types in banana (Musa spp.) at early growth stage
1999
Engelborghs, I. | Swennen, R. (Katholieke Univ. Leuven (Belgium). Lab. of Tropical Crop Improvement)
The "amplified fragment length polymorphism" or AFLP technique was optimised for banana (Musa spp.). This technique, based on the selective amplification of restriction fragments without prior sequence knowledge, allows the detection of many polymorphisms in a rather fast way. An automated sequencer and fragment "managing" software was used for fragment separation and fluorescent detection, and analysis, respectively. Banana plants are routinely propagated in vitro although somaclonal variation remains a serious bottleneck. Early detection of off-types is necessary before in vitro plantlets are field transplanted. The most frequent type of variation is dwarfness. AFLP profiles of dwarf-types of five cultivars "Cachaco enano", "Dwarf Cavendish", "Curare enano", "Figue Rose naine" and "Prata ana" were compared with their normal-sized plants. The pre-amplified DNA was used as a template for a selective amplification step with four to five selective nucleotides. Depending on the primer combination (34 tested) and the cultivar, up to 25/ polymorphism was detected between the dwarf and normal banana type.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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