Induction of protective immunity in chickens immunised with plasmid DNA encoding infectious bursal virus antigens
1999
Fodor, I. | Horvath, E. | Fodor, N. | Nagy, E. | Rencendorsh, A. | Vakharia, V.N. | Dube, S.K.
Direct DNA inocultions were used to determine the efficacy of gene immunization of chickens to elicit protective immune responses against infectious bursal disease virus (IBDV). The vp2 gene of IBDV strains GP40 and D78, and the vp2-vp4-vp3 encoding segment of strain D78 were cloned in an expression vector which consisted of human cytomegalovirus (HCMV) immediate early enhancer and promoter, adenovirus tripartite leader sequences and SV40 polyadenylation signal. For purification of vaccine-quality plasmid DNA from E.coli, an effective method was developed. Chickens were vaccinated by inoculation of DNA by two routes (intramascular and intraperitoneal). Two weeks later, chickens were boosted with DNA, and at 2 weeks after boost, they were challenged with virulent IBDV strain.
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