Practical preparation of K-252a from a fermentation solution
1998
Kino, M. (Kyowa Hakko Kogyo Co. Ltd., Hofu, Yamaguchi (Japan). Hofu Plant) | Shono, K. | Nishimura, T. | Nagamura, S.
We developed a practical preparation procedure for K-252a by methylating K-252b on an industrial scale. The water-insoluble K-252a, which was present in the cell mass, was converted to the water-soluble K-252b Na salt in an alkaline solution. The obtained K-252b was mutilated with dimethylsulfate in the presence of potassium carbonate in dimethylacetamide. We have already used this method to manufacture 90 kg of K-252b from the fermentation broth, and regenerated 65 kg of K-252a from K-252b
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