Cytological study in root tip cells in four cultivars of Sorghum bicolor
1997
Can, N.D. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Yoshida, T.
Several enzyme treatments for cytological observations were tested in order to characterize chromosome configurations in sorghum root tip cells. Treatments with enzyme containing 4% cellulase and 2% pectolyase Y-23 at 37 degrees C for one or two hours provided high quality for slide preparations with darkly and clearly stained chromosomes, and made possible for counting and photomicrograph. Chromosome number of 2n=20 was predominant in the majority of mitotic cells of root tips in all four cultivars. A few tetraploid cells and haploid cells were also observed in this study. The variations in chromosome size was detected in four cultivars studied. The length of chromosomes ranged from 2.5 to 5.5 mu-m. The length of small chromosomes varied from 1.25 mu-m. These observations confirmed earlier findings that the variations of chromosome morphology in several sorghum species existed
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