Simultaneous determination of 21 N-methylcarbamates and 12 metabolites in agricultural products by HPLC
1998
Tsumura, Y. (National Inst. of Hygienic Sciences, Osaka (Japan). Osaka Branch) | Nakamura, Y. | Yoshii, K. | Tonogai, Y. | Higo, M. | Shibata, T.
Twenty-one N-methylcarbamates (NMCs) and 12 metabolites or isomers in agricultural products were simultaneously determined. As extraction was accomplished with ethyl actate instead of dichloromethane, NMCs possessing a thioether group were partly oxidized to sulfoxides. They were quantified as total amount of oxidized and non-oxidized compounds. NMCs in food samples were extracted with acetone, then the acetone was evaporated off and sodium chloride was added before extraction with ethyl acetate. The extract was evaporated and the residue was partitioned between acetonitrile and n-nexane. The acetonitrile layer was evaporated and the residue was cleaned up on a Sep-PakR aminopropyl cartridge. NMCs were determined by HPLC with post-column reaction and fluorescence detection. Recoveries of NMCs from 10 kinds of agricultural products including rice, onion and apple were 56.9 to 108.2 %, except for butocarboxim sulfoxide, at fortification levels of 0.1 to 1 ppm. Detection limits we
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