Inhibitory effect of green tea on injury to a cultured renal epithelial cell line, LLC-PK1
1997
Yokozawa, T. (Toyama Medical and Pharmaceutical Univ. (Japan)) | Dong, E. | Chung, H.Y. | Oura, H. | Nakagawa, H.
When cells from a cultured renal epithelial cell line, LLC-PK1, were cultured under hypoxic conditions (oxygen concentration of 2% or less) before reoxygenation was applied (85% air, 5% CO2), the leakage of lactate dehydrogenase (LDH) into the medium increased. This phenomenon was inhibited in the presence of dimethyl sulfoxide, a hydroxyl radical scavenger, suggesting the involvement of free radicals. Such oxidative stress was significantly inhibited by a green tea extract, and more patently by a tannin minute. On the other hand, under ordinary culture conditions (85% air, 5% CO2), there was cell injury, although the LDH leakage was less than that under hypoxia/reoxygenation, and such injury was inhibited by the green tea extract and the tannin mixture
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