Identification of some sibling species complex of fruit flies by electrophoresis techniques
1997
Anchalee Saelee | Visut Baimai | Orawan Satayalai (Mahidol Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biology)
True fruit flies (Tephritidae:Diptera) are one of the most economically important agricultural pests throughout the world. The female oviposites her eggs via a long extendible ovipositor into fleshy tissues of fruits and/or the flower heads. The larvae hatch from the eggs and feed on the fruits, thereby resulting in the production of low quality fruits. One of the problems in previous studies is taxonomic confusion due to overlapping external morphological characteristics, especially in the sibling species complex. Horizontal polyacrylamide gel electrophoresis of twelve enzyme systems comprising of fifteen loci were used to separate some sibling species complex. The relative mobilities of enzyme at specific loci in this study can be used as genetic markers for species identification. The diagnostic banding patterns of three species of the Bactrocera dorsalis species complex correlates with the morphological characteristics and host plants. Three species of Bactrocera tau complex, the other sibling species, could be separated base on the combination of bandiang patterns of different enzyme loci. Therefore, electroretic technique is a useful tool for separation of sibling species and closely related species of fruit flies in Thailand.
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