The search for genetic resources of opium poppy (Papaver somniferum) with high thebaine content and the development of a screening method
2001
Havel, J. | Kolovrat, O. (Oseva Pro, Opava (Czech Republic). Vyzkumny Ustav Olejnin) | Kamenikov, L. | Bechyne, M.
Thebaine is an important source for the production of non-addictive analgesics in the pharmaceutical industry. The aim of the project was to develop a screening method for the determination of alkaloid composition in poppy and to find genetic resources of opium poppy (Papaver somniferum) with high thebaine content. A modified thin-layer chromatography appeared to be the most suitable method for the preliminary determination of alkaloid composition. This method was applied to breeding material and to varieties from the collection of poppy genetic resources. In the material from 1998 there were approximately 9 % of plants with higher percentage of thebaine. In the material from the years 1999 and 2000 the proportion of such plants was about 15.5 % and 11 %, respectively. Most of the plants had besides thebaine also a high content of other alkaloids, predominantly morphine. Five plants were rich only in thebaine and one plant had a high content of codeine. The stability of these traits will be tested further.
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