Glutathione Contents in Various Plant Cell Lines
2000
Lee Joung-Eun | Ahn Young-Ock Korea Research Institute of Bioscience and Biotechnology, Taejon (Korea Republic)
We investigated the levels of glutathione (GSH) and its oxidized form (GSSG) in 24 cell lines derived from various plant species to understand the antioxidative mechanism in plant cell cultures. The total glutathione content was 98 absolute value 27 micro g/g cell fresh wt, showing a slight difference in plant species. The average content of GSH and GSSG was 72 absolute value 20 and 26 absolute value 10 micro g/g cell fresh wt, respectively. The average GSH content in plant cell lines occupies approximately 73% in total glutathione. During the suspension cultures of Scutellaria baicalensis, one of the plant species we tested, the GSH content decreased in proportion to the cell growth during the exponential growth stage, showing the low level at the stationary growth stage (84 micro g/g cell fresh wt), whereas the GSSG content increased to the stationary growth stage (31 micro g/g cell fresh wt). The results suggested that the ratio of GSH and GSSG should be involved in the cell growth and antioxidative mechanism in cultured cells.
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