Genetic diversity analysis using microsatellite markers for identification and selection of inbred lines in hybrid maize breeding program
2001
Coronado, N.B. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding) Guzman, P.S. | Hautea, D.M. | Josue, A.D.L.
Knowledge of heterotic pattern makes hybrid maize breeding more defined and systematic heterotic patterns can be established using diallel analysis but the procedure is tedious, time consuming and costly, particularly if many lines or populations are involved. Molecular markers such as microsatellite have been used effectively to measure genetic diversity in many crops including maize. The extent of genetic diversity between parental lines have been associated with heterotic performance. Furthermore, genetic diversity analysis of inbred lines to be used as parents may be used to reduce the number of lines required for testing and the time needed to establish heterotic patterns. In this study, 33 yellow and 47 white inbred lines currently used in the IPB-CA-UPLB breeding program were fingerprinted using 60 selected SSR or microsatellite markers. Diversity analysis placed the yellow and the white inbred lines into four distinct clusters. Based on the genetic distances estimated among the inbred lines tested, 10 yellow and white lines were selected and used to generate diallel combinations for possible establishment of heterotic groupings
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