Binding of extracellular matrix molecules by staphylococci from wild herbivores
2002
Styriak, I. | Laukova, A. (Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat) | Ljungh, A.
Nine strains of staphylococci were examined for their antibodic resistance and for binding of seven extracellular matrix (ECM) molecules (bovine mucin, porcine mucin, porcine fibronectin, bovine fibrinogen, fetuin, bovine lactoferrin, and heparin) immobilized on latex beads in the particle agglutination assay. All 9 strains were resistant to bacitracin. Four strains displayed resistance to 5 of 13 antibiotics tested. Different binding capability between individual strains were observed. There were strains binding several ECM molecules (as S. warneri SW6 and S. epidermidis SE14) as well as strains which bound only one (S. aureus SA11) or no ECM molecule (S. saprophyticus SS16) of seven molecules tested. While some ECM molecules, e.g. porcine fibronectin and heparin, were bound by most of the strains, others (e.g. mucins) were bound only by 1 or 2 strains. Some strains bound these substrates weakly, however, other strains displayed strong binding, especially of heparin and porcine fibronectin. The preincubation of bacteria for 1 hour at room temperature with a protein used subsequntly in the PAA completely prevented the agglutination reaction, thus indicating the specificity of the assay.
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