Influence of co-culture with oviductal epithelial cells on in vitro maturation of canine oocytes
2002
Bogliolo, L. ((Institute of General Pathology, Sassari (Italie). Pathological Anatomy and Veterinary Obstetrics-Surgery Clinic, Obstetrics Section )) | Zedda, M.T. | Ledda, S. | Leoni, G. | Naitana, S. | Pau, S.
The process of oocyte maturation in the canine species is unique among mammals: oocytes are immature at ovulation and the resumption and progression of meiotic maturation occur in the oviduct. This study was performed to investigate (i) the effect of co-culture with infundibulum and ampullar oviductal epithelial cells on the in vitro maturation of canine oocytes and (ii) the culture time necessary to reach full meiotic maturation. For this purpose the oocytes, collected from the ovaries of bitches undergoing ovariectomies, were divided into three groups and cultured for 48 and 72 h with the following systems: (A) TCM 199 + 10 per cent oestrus bitch serum + FSH (0.1 IU m/L), LH (0.1 IU/mL ) + progesterone (1 microg/mL) + oestradiol (1 microg/ mL) + cysteamine (100 microM)(B) medium A plus infundibulum cells(C) medium A plus ampullar cells. Infundibulum and ampullar cells were recovered from the oviducts of bitches at the oestrus stage of their cycle. The results showed that after 48 h of incubation, a significantly higher meiotic resumption ( P 0.01) was observed in the oocytes cultured with infundibulum (59 per cent) and ampullar cells (60.0 per cent), than in the control group (40.0 per cent). There was also a significantly ( P 0.01) higher meiotic progression to the MII in systems B and C (15.6 per cent and 16.7 per cent) than in system A (4.0 per cent). After 72 h of culture, the percentages of meiotic resumption and progression were unchanged. These results showed that both the infundibulum and the ampullar oviductal epithelial cells positively influence the meiotic resumption and progression of canine oocytes and that 48 h are sufficient for the completion of nuclear maturation
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل National Institute for Agricultural Research