Effect of physical, physiological and genetic factors on callus induction, differentiation and regeneration of winter triticale (xTriticosecale Wittm.)

Marcinska, I.; Wedzony, M.

Effect of physical, physiological and genetic factors on callus induction, differentiation and regeneration of winter triticale (xTriticosecale Wittm.)

2002

Marcinska, I. | Wedzony, M.

Callus was initiated from immature embryos of winter hexaploid triticale cultivars Bogo, Moniko, Moreno, Newcale, Salvo and Vero. Salvo was used in its original version (cytoplasm Triticum aestivum) and as alloplasmic (fertile) inbred line (B11) with Triticum timopheevi cytoplasm (SalvoTt). The MS induction medium was supplemented with 4 mg/cm3 of dicamba. Regeneration was performed on hormone free MS medium. In this study, the influence of genotype, embryo size and cold treatment during the induction phase on the culture efficiency were determined. Calli fresh weight, their differentiation pattern and plant regeneration rate were evaluated.

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