Screening of RAPD [Random Amplified Polymorphic DNA] markers for the detection of foodborne pathogens
1998
Perez, M.T.M. (Philippines Univ. Los Banos, College, Laguna (Philippines). National Inst. of Molecular Biology and Biotechnology) Calapardo, M.C. | Ebora, R.V. | Elegado, F.B. | Garcia, M.R.C. | Guerra, M.A.R.V. | Mercado, M.A. | Ramirez, T.J. | Sapin, A.B. | Tambalo, R.D. | Brown, M.B. | Papa, M.J. | Alday, A.R. | Dancel, L.A. | Tobias, M.C. | Mercado, S.M.
A total of 242 isolates of several foodborne pathogens namely Escherichia coli, Salmonella, Staphylococcus aureus, Vibrio cholerae, Yersinia enterocolitica, Shigella sp., Listeria monocytogenes and Bacillus cereus were collected from hospitals and research institutions. Genomic DNA were covered from purified isolates using several procedures and used as templates for the screening of RAPD primers in DNA ampilification reactions. Potential RAPD markers were identified for the detection of some foodborne pathogen. Primers BIOTECH Code 10, 16 and 18 generated 0.9 kb, 2.6 kb and 2.3 kb bands, respectively in most Salmonella strains tested. A single, intense 1.4 kb band was generated in Staphylococcus aureus using BIOTECH Code 14. This primer also differentiated S. aureus from the other genera of foodborne pathogens. Primer BIOTECH Code 07 differentiated E. coli from Salmonella by generating a 1.3 kb band in E. coli strains tested. Primer BIOTECH Code 15 and Biotech Code 19 amplified a single intense band 2.4 Kb and 1.1 kb, respectively, in two Shigella strains tested. Further testing of these potential RAPD markers for reproducibility on different batches of genomic DNA are currently underway
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