Induction and evaluation of somaclonal variation in potato (Solanum tuberosum L.)

Nasrin, S. . Biological Research Div.; Hossain, M.M.; Khatun, A.

Induction and evaluation of somaclonal variation in potato (Solanum tuberosum L.)

2003

Nasrin, S. (BCSIR Labs., Dhaka (Bangladesh). Biological Research Div.) | Hossain, M.M. | Khatun, A.

Callus was induced from nodal internodal explants of potato Cvs. Multa and Diamant through the proper adjustment of auxin and cytokinins combinations. Callusing response was the best in MS supplemented with 1.0 mg L-1 NAA + 1.0 mg L-1 BA and 1.0 mg L-1 NAA+ 0.5 mg L-1 BA. The callus developed from both nodal and internodal segments induced to develop shoot when subculture on to MS containing same growth regulator formulations. MS containing 3 mg L-1 KIN with 1.5 mg L-1 NAA was the most responsive medium for shoot regeneration. Plants regenerated through callus culture after transplanting in field displayed somaclonal variation for plant height, number of laves/plant, number of tubers/plant and tuber weight/plant. This variability may incorporate for the potato breeding program.

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