Effect of some factors including irradiation on the Ergot alkaloids production by members of Penicillium
2003
Moussa, L.A.A. (National Center for Radiation Research and Technology, Cairo (Egypt). Microbiology Dept.)
Six hundred isolates belonging to 30 species of the genus Penicillium were screened for ergot alkaloids production. Ergot alkaloids were confined to 67 isolates of 17 species. P. corylophilum (isolate No. 2760) produced 750 mg of ergot alkaloids per litter of culture medium and was selected as the experimental organism. Biosynthesis of alkaloids was markedly affected when the experimental organism was cultured on basal liquid medium of different types and levels of carbon and nitrogen sources, pH values, phosphate concentration and incubation temperature. The mycelial dry weight of P. corylophilum and ergot alkaloids accumulation decreased by increasing the irradiation dose levels and the fungus was completely inhibited at 3.0 kGy. Chromatographic and chemical analysis showed that mycelial extract of P. corylophilum (isolate No. 2760), cultivated as static culture under optimal conditions for alkaloids formation produced 1.380 g/l. Alkaloids analysis of the crude extract clearly show that it is composed of three components, namely ergokryptine, agroclavin and elmoclavine. The identity of the compounds was confirmed by high performance liquid chromatography and ultraviolet and infrared analysis.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Research Centre
