Identification of oilseed rape cultivars using AFLP markers
2002
¬urn, V. | Sakova, L. | Sobotka, R. (South Bohemia Univ., ¬eske Budejovice (Czech Republic). Agriculture Fac.) | Ovesna, J. (Research Institute of Crop Production, Ruzyne (Czech Republic))
A new type of molecular marker, fluorescence-based AFLP, was evaluated for its ability to identify oilseed rape cultivars. Each of the six tested AFLP combinations detected polymorphisms, the best combination (M-CAA/E-ACT) had 26% of polymorphic peaks from a total of 90 peaks and could distinguish analysed cultivars, and 4 out of 5 DH lines. The results presented here show that florescence-based AFLP was, for the purposes of oil seed rape cultivar fingerprinting, a very suitable approach.
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