The occurrence of Salmonella spp. in natural environment confirmed by biochemical and molecular methods
2002
Proksova, M. | Ferianc, P. | Harichova, J. (Slovak Academy of Sciences, Bratislava (Slovak Republic). Institute of Molecular Biology) | Morgonova, E.
Salmonella spp. is pathogenic bacterium often detected in sewage, freshwater and groundwater. This genus of bacteria can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. The occurrence of Salmonella spp. was investigated in the surface water of the Danube river in three sampling sites and of the V h river in one sampling site from August 2001 to April 2002. A total of 36 water samples were collected. Before the isolation, water samples were pre-enriched in buffered peptone water and enriched in Rappaport-Vassiliadis medium. The isolations were realised in brilliant green agar with phenol red, xylose lysine deoxycholate agar and bismuth sulphite agar. Colonies with typical growth and Gram staining negative, cytochrome-oxidase negative, positive for production of hydrogen disulphide and lysine decarboxylase were considered as Salmonella spp. These colonies were detected in 20 samples (55.5%). The obtained isolates were identified by further biochemical tests, and confirmed by serotyping with polyvalent O antisera, and by the polymerase chain reaction (PCR) using primer pairs targeted to fimC gene. PCR reactions have confirmed only 15 isolates as Salmonella spp. In addition, the presence of Salmonella spp. was also confirmed by PCR reaction directly in water samples of the Danube river. Furthermore microbial indicators of faecal pollution (total coliforms and thermotolerant coliforms) were investigated. No correlation between numbers of microbial indicators and occurrence of microbial indicators and occurrence of Salmonella spp. was found
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