Method for the microscopic image compilation of the potato tuber's cellular structure
2003
Gancarz, M. | Konstankiewicz, K. | Krol, A. | Pawlak, K. (Polish Academy of Sciences, Lublin (Poland). Inst. of Agrophysics)
This study presents a method of compiling flat microscopic images of the parenchyma tissue structure of potato tubers in their natural state. An optical confocal microscope, equipped to precisely shift an object along an X-Y plane, was used. Parameters of the cell structure for single images and images compiled of 16 single images were determined. The experiment was carried out on inner and outer cores of the potato varieties Danusia, Kuba, Mila, Triada. The number of cells obtained by the compilation method was higher by 40 % in comparison with single images. Besides this, a larger surface of the tissue may be observed. The number of cells obtained is sufficient to undertake statistical analysis of cell structure parameters. In Danusia, Kuba and Triada the inner core was characterised by smaller cells than the outer core, while this relation was reversed in the variety Mila. The method mentioned above can be applied to each microscopic image of good quality.
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