[Effect of Gamma irradiation on forming variation of Catharanthus roseus (L.) Don clones]
2000
Syukur, S. (Universitas Andalas, Padang (Indonesia))
Clonal variation have been found in Catharantus roseus plant after gamma irradiation. Several doses have been used to produce clonal variation. The most effective doses used to perform better clonal variation was 20 krad. About 103 seeds have been irradiated for every radiation treatment, but only several clones were grown better than the wild type. We have success to get (M) seeds from the expected mutant. The seeds from selected mutant are bigger when compare to the wild type and growth better on medium containing 5-methyl Tryptophan (5-MT). The chlorophyll content is higher (almost twice) as compared to the wild type. Further experiment continue to do in vitro culture in order to develop embryonic callus from leaf tip and leaf base. Several manipulation of auxin and cytokinin have been used to differentiate the callus formation. Modified MS medium with kinetin and cytokinin (10:1) can induce globular embryo like structure. Dragendrof alkaloid reagent were used to determine high alkaloid clones from the expected mutant. TLC analysis from callus mutant showed 3 clear bands with subsequence Rf about 0.22, 0.41, 0.58 while control showed two smearing bands at 0.21 and 0.52.
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