Genetic similarity among five species of Saccharum based on isozyme and RAPD markers
2001
Hemaprabha, G. | Rangasamy, S.R.Sree(Tamil Nadu Agricultural University, Coimbatore(India). Center for Plant Molecular Biology)
Genetic relationship among Saccaharum species clones was assessed using six isozyme systems and thirteen RAPD primers. Multimeric isozyme profile and polymorphic RAPD markers reflected the genetic complexity of the genus. Clones should be distinguished fro one another only based on the esterase profile through species affinities could not be detected using this system. Clonal distinction and intraspecies could be under stood using a combination of six isozyme systems studied. Low genetic dissimilarity index within Saccharum and overlapping of isozyme pattern of a few clones with other species were suggestive to continous variation across Saccharum species. Species sffinities as revealed through dendrogramconstructed based on Phylogenetic Analysis Using Parsimony indicated closeness between S. barberi and S. sinense and between S. officinarum and S. robustum clones. Relationship between S. spontaneum with S. barberi and S. sinense observed through RAPD analysis supported S. spontaneum based origin of the species. The results of isozyme and RAPD analyses were comparable and agreed to the classically derieved evolutionary affinities of the genus.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Indian Council of Agricultural Research