Performance of oil adjuvant combined vaccine cintaining FMD, Rabies, Pasteurella multocida and Clostridium chauvoei antigens

Combined vaccines are attactive to farmers since the stress to their animals is less, as fewer musterings are necessary and also for economic reasons. Many developing countries in South East Asian region face serious economic losses due to important infectious diseases like foot and mouth diesease (FMD), haemorrhagic septicaemia (HS) and black quarter (BQ). In addition diseases like rabies cause mortality and occurs in the form of outbreaks . Therefore,thrust areas for control and eradication of infectious diseases are disease surveillance and prophylactic vaccination. Several workes attempted to increase the duration of immunity using oil adjuvant formulations against FMD and HS. The present experiment was designed to study the performance of oil adjuvant vaccine containing FMD, Rabies, Pasteurella multocida and clostridium chauvoeiantigens in comparison with individual component vaccines. Binaryethylenimine (BEI) incativated antigents of O,A,C and Asia 1 strains of FMD and CVS strain of rabies virus were concentrated by 20 times and formulated with equal volumes of Montanide ISA 206 (SEPPIC) and homogenised using IKA homigeniser to form water-in-oil-water emulsion (Double emulsion). Chloroform was added as preservative at 0.05 v/v and the dose volume was 2 ml. each of FMD and rabies vaccine. Formalin inactivated, purified and 20 times concentrated antigens of pasteurella multocida and closridium chauvoei were used to formulate the oil adjuvant vaccine.The dose volume of HS+BQ vaccube was 2 ml. The combined oil adjuvant vaccine with antigents of FMD, Rabies, Pasteurella multocida and clostridium chauvoei was formulated with the same antigen payload as individual with the sane antigen payload as individual component vaccines as described earlier with a final dose volume of 4 ml. Crossbred calves of 12 to 16 months age seronegative for FMD, Rabies, Pasteurella multocida and clostridium chauvoei were used in the experiment. Four groups of five animals each were vaccinated intramuscularly with different formulations of vaccine i.e.,FMD, HS+BQ, Rabies and FMD+ HS + BQ + Rabies. The animals vaccinated with FMD alone and FMD + HS + BQ + Rabies vaccines were given booster dose on 21 days post-vaccination (dpv) with FMD aqueous vaccine. Five animals were kept as unvaccinated controls. Serum separated from blood samples collected on 0.21 and 90 dpv were inactivated at 56 degree C for 30 minutes and stored at -20 degree C till further use. The serum antibody titres to FMD antigens were messured by micro-serum neutralisation test (SNT), to Rabies antigen by Rapid Fluorescent focus inhibition Test (RFFIT), to Pasteurella multocida and clostridium chauvoei by ELISA. Standard statistical methods were used to compare the data on serological response of calves to different vaccines.