High-Level Expression in Escherichia coli of Alkaline Phosphatase from Thermus caldophuilus GK24 and Purification of the Recombinant Enzyme
2003
Lee, J.H. | Cho, Y.D. | Choi, J.J. | Lee, Y.J. | Hoe, H.S. | Kwon, S.T. (Sungkyunkwan University, Suwon, Republic of Korea) | Kim, H.K. (Super Bio Co., Ltd, Suwon, Republic of Korea)
High-level expression of Thermus caldophilus GK24 alkaline phosphatase was achieved in Escherichia coli using the pET-based expression plasmids, pEAP1 and pEAP2. In the case of plasmid pEAP2, the signal peptide region of Tca APase was replaced by the PelB leader peptide of expression vector pET-22b.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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