Eradication of orchid virus by thermotherapy and meristem culture for disease-free Oncidium
1997
Jaruwan Chartisathian | Surapee Kiratiya-angul | Sirilux Losawas (Department of Agriculture, Bangkok (Thailand). Plant Pathology and Microbiology Div.)
Widespread to viral disease caused by Cymbidium Mosaic Virus (CyMV) and Odontoglossum Ring Spot (ORSV) have been reported to reduce the products in orchid culture. The general method of disease-free plant production were thermotherapy and meristem culture. But the thermotherapy was not suitable for oncidium orchid because young bulbs changed to yellow and 17 from 20 meristem tips were dead after one week of hot air (40 deg C) treatment. The others 3 shoots were dead after culture. When using only meristem culture to eradicated of CyMV and ORSV. It was found that the 8 explants of apical meristem and 2 explants of auxiliary bud meristem of total 107 explants of both (0.2-0.3 mm size) could survive and growth. After ELISA tests for CyMV and ORSV, the 2 explants auxiliary bud meristem showed both positive test but only 2 in 8 explants of apical meristem showed negative CyMV and positive ORSV and only one of apical meristem both negative. The only one that free from CyMV and ORSV could be proliferated in Vacin&Went media through the process of orchid mass propagation. The 10 percent of total number were ELIZA test and all sample showed both negative.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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