Detection of antibiotic residues in milk with a new screening test kit, KS-9
1998
Kriengsag Saitanu | Thongchai Chalermchaikit (Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Science. Dept. of Microbiology)
New microbial inhibition assay test kit, KS-9, was used to detect inhibitor substances in 431, 200, 565 and 1000 samples of farm canned milk (FM), tanker milk (TM), pasteurized milk (PM), and UHT-milk (UM), respectively. In conjunction to KS-9, other 4 commercial test kits and disk assay method were performed on randomized milk samples. Randomized milk samples from KS-9 positive and KS-9 negative tests were primary confimation by Charm II Test. The result indicated that KS-9 was the most sensitive test for detecting of the inhibitor substances in milk samples; showing 36.9, 62.0, 42.5 and 1.8 percent of FM, TM, PM and UM respectively. The Charm II Test found that 67.5, 71.4, 98.0 and 61.1 percent of KS-9 positive samples of FM, TM, PM and UM, respectively, were contaminated with antibiotics which the beta-lactams and tetracyclines were the majority identified. While 50.0, 61.8, 49.2 and 37.5 percent of KS-9 negative samples of FM, TM, PM and UM, respectively, were found to be contaminated with antibiotics, especially tetracycline group. This study indicated that raw milk contained high percentages of natural inhibitors and antibiotic residues. PM were also highly contaminated with antibiotic residues. KS-9 was highly sensitive to detect antibiotic residues in PM but gave high false positives when tested on non-heated raw milk and UHT milk.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Kasetsart University