Screening of microorganisms suitable for lysine production
1997
Somchai Siriphok | Khachinat Phothiwetchakun | Phonphan Loetthawisin | Sumali Luangsakun (Srinakharinwirot Unvi., Bangkok (Thailand). Prasarnmit Campus. Faculty of Science. Dept. of Biology)
Isolation of amino acid producing bacteria from soil samples was done by cultivation the bacteria on minimal medium with 100 micro g/l cycloheximide. The 342 isolates of bacteria were isolated from 82 soil samples. These bacteria were screened for lysine production in the production medium of the following composition (g/l):glucose 50,NH4Cl 10, tryptone 4, KH2PO4 0.5, K2HPO4 0.5, MgSO4.7H2O 0.25, FeSO4.7H2O 0.01, MnSO4.H2O 0.01, yeast extract 1, CaCO3 5. The culturing of these bacteria was carried out on a rotary shaker with 200 rpm at room temperature (in the range about 30-35 deg C) for 3 days. Lysine in the culture supernatants was determined by paper chromatography method. The result showed that there are 52 isolates which can produce lysine. Among these 52 isolates there are 9 potential lysine producers (KT7.1, KT9.3, KT9.4, KT21.1, KT21.2, KT22.2, KT25.1, NK1.5 and PL2.2) which produced lysine ranged from 0.43 to 0.59 g/l of the medium volume. The studies on morphology and Gram's stain of these bacteria revealed that all of these isolates are Gram's positive, rod-shaped and endospore-forming bacteria. Therefore, these bacteria were considered to be the genus Bacillus.
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