Preliminary study of the competition and persistence of applied Bradyrhizobia strains using reporter gene in soil
1998
Teaumroong, N. | Teaumtisong, M. | Manassila, M. | Boonkerd, N. (Suranaree Univ., Nakhon Ratchasima (Thailand). Inst. of Agricultural Technology. School of Animal Production)
To investigate the behavior of Bradyrhizobia inocula in the field, Bradyrhizobium sp. TAL 1000 isolated from Arachis hypogaea and B. Japonicum TAL379 from Glycine max were choosen in this study. Both of bradyrhizobial strains were conjugated with E. coli Strain S 17-1 lambda pir/pUT mTn 5SS gus A31. The conjugants were inoculated in soybean seeds along with their host. After 30 days, the seedlings were removed and stained with X-gluc. The nodule occupancy was determined in various sandy soil samples. The percent of nodule occupancy was analyzed by number of blue colour developed in the nodule tissue. The highest number of occupied nodules was found in the soil collected from Eucaryptus field. The highest competition condition was most likely occured in the soil sample from teak field which related to low amount of organic matter.
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